Author(s): Safila Naveed* and Nimra Waheed

Objective:Ciprofloxacin is an established broad-spectrum fluoroquinolone antibiotic displaying activity against both Gram-positive and Gram-negative bacteria pathogens. It acts principally by inhibiting DNA-gyrase and topoisomerase IV present in bacterialcells which are responsible for reproduction of DNA of bacteria. A rapid, simple, accurate, and economical least time consuming spectrophotometric method has been developed for the assay of Ciprofloxacin and then compare it with the  assay of different brands available in Karachi, Pakistan.  A sample of drug was dissolved in water to produce a stock solution containing ciprofloxacin.Methods:Samples of ground tablets of different brand  were  taken  and  their  diluted  solutions  of  100ppm,  50ppm  ,  25ppm,12.5ppmand  6.25ppm  were  prepared  with  the  same  solvent.  The  assay  was  determined  by measuring the absorbance of stock solution against the solvent blank and comparing with the absorbance of available brands ofciprofloxacin at the wavelength of 278 nm by spectophotometer. Results:The percent assay is calculated and the correlation coefficient for different brands was found to be 0.9998 for (A), 0.9992 for (B), 0.9996 for (C) and CYCIN (D), 0.9989 for (E) and 0.9986 for (F) which are within the limit.  Conclusion:Thus we can conclude that the method can be applied for the routine QC quantitative analysis of ciprofloxacin in active and tablet formulation.

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