Safila Naveed* and Nimra Waheed
Objective:Ciprofloxacin is an established broad-spectrum fluoroquinolone antibiotic displaying activity against both Gram-positive and Gram-negative bacteria pathogens. It acts principally by inhibiting DNA-gyrase and topoisomerase IV present in bacterialcells which are responsible for reproduction of DNA of bacteria. A rapid, simple, accurate, and economical least time consuming spectrophotometric method has been developed for the assay of Ciprofloxacin and then compare it with the assay of different brands available in Karachi, Pakistan. A sample of drug was dissolved in water to produce a stock solution containing ciprofloxacin.Methods:Samples of ground tablets of different brand were taken and their diluted solutions of 100ppm, 50ppm , 25ppm,12.5ppmand 6.25ppm were prepared with the same solvent. The assay was determined by measuring the absorbance of stock solution against the solvent blank and comparing with the absorbance of available brands ofciprofloxacin at the wavelength of 278 nm by spectophotometer. Results:The percent assay is calculated and the correlation coefficient for different brands was found to be 0.9998 for (A), 0.9992 for (B), 0.9996 for (C) and CYCIN (D), 0.9989 for (E) and 0.9986 for (F) which are within the limit. Conclusion:Thus we can conclude that the method can be applied for the routine QC quantitative analysis of ciprofloxacin in active and tablet formulation.