Abstract

RP-HPLC METHOD FOR DETERMINING THE LEVELS OF GUAIPHENESIN AND PHENYLEPHRINEIN PHARMACEUTICAL DOSAGE FORMS

Author(s): MALLADI BABU*, PAVAN KUMAR CHADALAWADA,S.MANOHAR BABU

A  simple,  precise  and  accurate  reverse  phase  high  performance  liquid  chromatographic  method  was  developed  for  the  simultaneous  estimation  of  guaiphenesin  and phenylephrine in their tablet dosage form. The chromatographic conditions were standardizedusing anInertsilcolumn,C18 (150x4.6 ID) 5μm with UV detection at 277nm , and the mobile phase consisted of sodium dihydrogenphosphate buffer: Acetonitrile (30:70).The retention times of guaiphenesin  and phenylephrine were About  3.497 min for  Guaifenesin  and  2.353min  for  Phenylephrine,  respectively.  The  calibration  curves  were  linear  with  correlation  coefficients  of  0.9987,  0.9988,  0.9981  and  0.9981  over  a concentration  range  of  4.0–24.0  μg/ml  for  guaiphenesin,  5.0–30.0  μg/ml  for  phenylephrine,  respectively.  The  proposed  method  has  been  validated  according  to  the  ICH guidelines and was successfully applied to estimate the levels of two drugs in a combined formulation with good accuracy and precision

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ISSN: 2320-3315

ICV :81.58

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