1. Situs Slot KayaMendadak88
  2. Situs Slot Online Terbaik Dan Terpercaya No 1
  3. Slot Pragmatic Play
  4. Situs Slot Online Terpercaya
  5. Kumpulan Situs Judi Slot Terpercaya
  6. Game Slot Online
  7. Judi Slot Online Jackpot Terbesar
  8. Situs Judi Slot Online Terpercaya 2021
  9. Slot Joker123
  10. Agen Slot Online Resmi
  11. Slot Deposit Pulsa
  12. Daftar Situs Judi Slot Terbaik Dan Terpercaya No 1
  13. AloJudi Slot
  14. KayaMendadak88 Slot
  15. Situs Slot Gacor 2022
  16. Judi Slot Online Jackpot Terbesar Gampang Menang
  17. Joker123 Slot
  18. Daftar Situs Judi Slot Online Deposit Pulsa Terbaik
  19. Mesin slot gacor 88
  20. Mesin slot
  21. Link Slot Online
  22. Slot Via Dana
  23. situs judi slot terbaik dan terpercaya no 1
  24. Daftar Situs Bo Slot Online Gacor Terbaik 2022
  1. Slot Online
  2. Judi Slot Online Jackpot Terbesar
  3. Daftar Situs Judi Slot Online Terpercaya
  4. Judi Slot Terpercaya
  5. Kumpulan Situs Judi QQ Online Terpercaya
  6. Slot Online Terbaru
  7. Situs Game Slot Online Terbaik
  8. Daftar Situs Judi Slot Online Gacor Gampang Menang 2022
  9. Situs Slot Deposit Pulsa dan Slot Online Terbaik
  10. Slot Hacker
  11. Situs Slot Online Terbaik
  12. Slot Gacor Gampang Menang
  13. Situs Judi Slot Online Gacor Terbaik 2022
  14. Situs Judi Slot Online Jackpot Terbesar
  15. Slot Online Terpercaya
  16. Game Slot Penghasil Uang
  17. Situs Slot Online Terbaik 2021
  18. Situs Slot Online Terbaik dan Terpercaya
  19. Situs Slot Terbaik
  20. Situs Judi Slot Terbaik Dan Terpercaya No 1

Abstract

PRODUCTION OF L-ARGINASE UNDER SSF AND ITS OPTIMIZATION

Author(s): RAHAMAT UNISSA, KOMATI BHANU REKHA, DAGGUPATI AMANI, SOWMYA KONAKANCHI, SARANYA ARUN KUMAR

Objective: The present study deals with the fermentative production of l-arginase under solid state fermentation technique .Various nutritional as well as physical parameters were altered in order to maximize the yield. Method: L-Arginase has received significant attention in recent years owing to its potential applications in medicine as an anticancer agent, as an efficient diagnostic agent and as a biosensor. Production of the enzyme was carried out by using various solid substrates such as wheat bran, tea dust, rice bran, ragi, maize bran, black gram, bread, red gram as solid substrates. Various factors such as incubation periods (24-168 hrs), p H (5-10), inoculum size (2.5- 15%), incubation temperature (20-450C), l-arginine concentration (0.5-3%), NaCl concentration (1-5%) etc. were altered and their effect on the production rate was noticed. Results: The maximum yield (66.08 IU/ml) of l-arginase by Idiomarina sediminium was obtained using tea dust as a solid substrate, initial pH 9.0, supplemented with maltose (1.0%), casein (1.0%), inoculated with 10% of inoculum and incubated at 37°C for 120 h. Conclusion: Solid state fermentation method can be adopted for large scale production of the enzyme for the therapeutic use in the future.

Get the App