1. Situs Slot KayaMendadak88
  2. Situs Slot Online Terbaik Dan Terpercaya No 1
  3. Slot Pragmatic Play
  4. Situs Slot Online Terpercaya
  5. Kumpulan Situs Judi Slot Terpercaya
  6. Game Slot Online
  7. Judi Slot Online Jackpot Terbesar
  8. Situs Judi Slot Online Terpercaya 2021
  9. Slot Joker123
  10. Agen Slot Online Resmi
  11. Slot Deposit Pulsa
  12. Daftar Situs Judi Slot Terbaik Dan Terpercaya No 1
  13. AloJudi Slot
  14. KayaMendadak88 Slot
  15. Situs Slot Gacor 2022
  16. Judi Slot Online Jackpot Terbesar Gampang Menang
  17. Joker123 Slot
  18. Daftar Situs Judi Slot Online Deposit Pulsa Terbaik
  19. Mesin slot gacor 88
  20. Mesin slot
  21. Link Slot Online
  22. Slot Via Dana
  23. situs judi slot terbaik dan terpercaya no 1
  24. Daftar Situs Bo Slot Online Gacor Terbaik 2022
  1. Slot Online
  2. Judi Slot Online Jackpot Terbesar
  3. Daftar Situs Judi Slot Online Terpercaya
  4. Judi Slot Terpercaya
  5. Kumpulan Situs Judi QQ Online Terpercaya
  6. Slot Online Terbaru
  7. Situs Game Slot Online Terbaik
  8. Daftar Situs Judi Slot Online Gacor Gampang Menang 2022
  9. Situs Slot Deposit Pulsa dan Slot Online Terbaik
  10. Slot Hacker
  11. Situs Slot Online Terbaik
  12. Slot Gacor Gampang Menang
  13. Situs Judi Slot Online Gacor Terbaik 2022
  14. Situs Judi Slot Online Jackpot Terbesar
  15. Slot Online Terpercaya
  16. Game Slot Penghasil Uang
  17. Situs Slot Online Terbaik 2021
  18. Situs Slot Online Terbaik dan Terpercaya
  19. Situs Slot Terbaik
  20. Situs Judi Slot Terbaik Dan Terpercaya No 1

Abstract

MOLECULAR METHODS FOR THE DIAGNOSIS OF PNEUMOCYSTIS JIROVECII IN IMMUNOCOMPROMISED IRAQI PATIENTS

Author(s): AZHAR A. F. IBRAHIM1, ISA S. TOUHALI*1,HAIDERN. DAWOOD

Objective: The    study  were  to:  Determine    the    possible  implication of  Pneumocystis  jirovecii    in  a  sample  of    immunocompromised  Iraqi  patients  and  determine    the  prevalence  of    Dihydropteroate  synthase gene    related  to  treatment and  prophylaxis  failures. Methods:This  study  included  a  total of  two hundred  (n=200)clinical samples from  100  immunocompromised patients as  70  Bronchoalveolar  lavage  ,  21sputum  samples and 9  pleural fluids,  with underlying   different  diseases. Control group  included 100  samples(50  Bronchoalveolar  lavage,  30  sputum  samples  and  20  pleural  fluids) from  immunocompetent  individuals  from  immunocompetent  individuals  were  collected from of in-and out patients who attended of  Medical AL-Imammian AL-Kadhmain City teaching Hospital / Baghdad, Baghdad teaching Hospital / Baghdad, Al-Zahra teaching Hospital / Wasit province and other private Laboratories, during the period from May-2014 to March-2015. The detection pneumocystosis by molecular methods. Result:The organism was detected in bronchoalveolar lavage, and/or sputum of immunocompromised patients only. The highest detection rate was observed for the mtLSUrRNA gene in 19  (19%)  samples,  15  (15%)    of  these  samples    of  immunocompromised  patients  consist  of  DHPS  genes.  Conclusion:Pneumocystis  jirovecii,  is  the  fundamental opportunistic infection among immunocompromised patients.  The primers target  of  mtLSUrRNA has the most specific and sensitive for the detection of P. jirovecii. In the present study showed the  DHPS  gene  has served as a selective drug linked with sulfa resistance and PCR amplification target.

Get the App