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Abstract

IN VITRO ANTIPROLIFERATIVE AND IN SILICO ACTIVITY OF RUBIADIN ISOLATED FROM ROOTS OF Rubia Cordifolia

Author(s): PADMAA M PAARAKH*1, DILEEPCHANDRA SREERAM2, SHRUTHISD3, SUJAN GANAPATHY PS4, VEDAMURTHY AB

Background:Rubia cordifolia  Linn.[  Rubiaceae],  commonly  known  as  Indian  Maddar  and  Manjistha,  is  used  as  medicine  for  treatment  of  various ailmentsin  Traditional System of Medicine of India.  Purpose:To isolate rubiadin and evaluate in vitro antiproliferative activity and in silico method. Material and Methods:Rubiadin was isolated from the roots of R.cordifolia. Rubiadin  was characterized by IR, 1H-NMR, 13C-NMR and Mass spectrum. Standardization of rubiadin was done also by HPLC fingerprinting.  In vitro antiproliferative activity was done using HeLa cell lines by MTT assay at different concentrations ranging from 20-100 μg/ml intriplicate and in silico docking studies using enzyme EGFR tyrosine kinase. Results:Fingerprinting of isolated rubiadin were done by HPLC method. The IC50value was found to be 56.63 ± 0.025 μg/ml in in vitro antiproliferative activity  in  HeLa celllines.  Rubiadin was  subjected  to  molecular  docking  studies  for  the  inhibition of  the enzyme  EGFR  tyrosine  kinase,  which  is  one  of  the targets  for  inhibition of  cancer  cells.  It  has  shown -7.07  kJ  mol-1 binding and -7.12  kJ  mol-1 docking energy with  two hydrogen  bonds.  Conclusion: Rubiadin has  shown  to possess in vitro antiproliferative activity and in silico studies

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