Mintage Journal of Pharmaceutical and Medical Sciences (ISSN: 2320-3315)

Author(s): ABDULKAREEM H.ABD1, MARWAH HASAN ABD2,FATMA ABDULHAMZA OBED3

The  aim  of  this  study  to  determinedin  vitro  effects  of  various  combinations  of  three  antimicrobials  (nitrofurantoin,  gentamicin  and  ciprofloxacin)  against  twenty  five E.  coliisolates and the role of plasmidDNA in antimicrobial resistance.Method: Seventy five E. coli isolates were collected from urine of patients with urinary tract infections in AL-Kadhimia and AL-Yarmook teaching hospitals in Baghdad for a period between 22/11/2012 to 15/3/2013,from these samples twenty five isolates were selected according to their pattern of the highest resistance as these showing multi-drug  resistances  and  tested  to  specify  their  minimum  inhibitory  concentration  for  (nitrofurantoin,  gentamicin  and ciprofloxacin. The  plasmid profile  for  the  twenty  five E.  coli isolates  were  studied  using  Pure Yield™ plasmid Miniprep system-Cat.#  A1220 –Promega-USA.  In  order  to  determined  the  presence  of  plasmid  for  antimicrobials resistance.Result  :Among  combinations  the  combination  of  nitrofurantoin  with  gentamicin  showed high  synergistic  effect  when  1/4+1/4  MIC  for  each  antimicrobial  were  used.  While combinations of nitrofurantoin with gentamicin and ciprofloxacin in some isolates showed additive effect when 1/2+1/2 MIC foreach antimicrobial were used. ), nitrofurantoin was  found  having  the  lowest  MIC  comparing  with  others.Extraction  of  plasmidDNA indicatesthe  presence  of  antimicrobial  resistance  plasmid in  (A6,  A37,  A32, and  A57) isolates.Conclusion:  Nitrofurantoin  had more  effect  on E.coli and with high  synergistic  effectin  combination with gentamicin against  resistant Ecoli.that  18 isolatesshow  synergistic effect, only2isolate show additive effect, while combination of nitrofurantoin andquinolon better to be avoidedthat in vitro show antagonist effect