Mintage Journal of Pharmaceutical and Medical Sciences (ISSN: 2320-3315)

Author(s): SR KOTRA1*, N PRUDVI2, KRA SADA SAI2, KK MANNAVA2, JB PERAVALI3, ANMOL KUMAR1, KRS SAMBASIVA RAO1, KK PULICHERLA

L –asparaginaseis known to have a therapeutic function and was used for the patients alleged from acute lymphoblastic leukemia (ALL). Out of different micro organisms, fungal samples isolated from a local soil sample, Pencillium sps. 02have utmost L-asparaginase activity. In the present study, Pencilliumsp. 02 was isolated and studied for enhanced  enzyme  production  with  the  help  of  statistical  methods. Initially,  all  nutritional  factors  were  selected  by  one  factor-at-a-time  method (171  IU/ml),  then  the consequence of every factor with respect to production of L-asparaginase was identified by taguchi design and the enzyme yield was increased to 1.073 folds (171 IU/ml to 183.5 IU/ml). Later, final optimization was carried out with response surface methodology (Central Composite Design) increased the yield of 1.065 folds (183.5 IU/ml to 195.5 IU/ml)