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Abstract

COMPARATIVE STUDY OF ANTIBACTERIAL ACTIVITY BETWEEN THE CITRUS LIMON (LEMON) & CITRUS AURANTIUM (BITTER ORANGE) EXTRACTS ON PSEUDOMONAS AERUGINOSA ISOLATES FROM BURNS INFECTIONS

Author(s): BASAM BASIM MOHAMMED, SOMAYA YASSIN NAJIM , ZAINAB OWAID SHATTI , ENAS IBRAHIM JASIM AND WISSAM ADNAN DARI

Aims: This study to the compared antibacterial activity of the Citrus Juices (lemon & bitter orange) in vitro by the well diffusion assay against Pseudomonas aeruginosa isolated from burned patients in Baghdad, Iraq. Materials and Methods : Freshly squeezed lemon and bitter orange juices diluted with water; Four different concentrations of each extract, (25%, 50%, 75%, 100%) were prepared of C. limon (lemon) & C. Aurantium (bitter orange) juices ; in order to check their the antibacterial activities against 10 isolates of Pseudomonas aeruginosa gram-negative bacteria by agar diffusion method with measurement of diameter of the zone of inhibition around the extracts.Results: The highest inhibition zone (45 mm) was observed with 100% concentration of C. limon on Pseudomonas aeruginosa , while the highest inhibition zone recorded by C. Aurantium was (34 mm). Less concentration used in C. limon and C. aurantium juices at 25% concentration had antibacterial effects on the bacteria isolates tested. Conclusions : The different concentrations of Citrus limon juice had an effective antibacterial activity more than citrus aurantium juice against all Pseudomonas aeruginosa isolates . The citrus fruits extracts have lesser side effects than the synthetic medicines used against pathogens & can be used in vitro to treat surface problems of the skin.

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