1. Situs Slot KayaMendadak88
  2. Situs Slot Online Terbaik Dan Terpercaya No 1
  3. Slot Pragmatic Play
  4. Situs Slot Online Terpercaya
  5. Kumpulan Situs Judi Slot Terpercaya
  6. Game Slot Online
  7. Judi Slot Online Jackpot Terbesar
  8. Situs Judi Slot Online Terpercaya 2021
  9. Slot Joker123
  10. Agen Slot Online Resmi
  11. Slot Deposit Pulsa
  12. Daftar Situs Judi Slot Terbaik Dan Terpercaya No 1
  13. AloJudi Slot
  14. KayaMendadak88 Slot
  15. Situs Slot Gacor 2022
  16. Judi Slot Online Jackpot Terbesar Gampang Menang
  17. Joker123 Slot
  18. Daftar Situs Judi Slot Online Deposit Pulsa Terbaik
  19. Mesin slot gacor 88
  20. Mesin slot
  21. Link Slot Online
  22. Slot Via Dana
  23. situs judi slot terbaik dan terpercaya no 1
  24. Daftar Situs Bo Slot Online Gacor Terbaik 2022
  1. Slot Online
  2. Judi Slot Online Jackpot Terbesar
  3. Daftar Situs Judi Slot Online Terpercaya
  4. Judi Slot Terpercaya
  5. Kumpulan Situs Judi QQ Online Terpercaya
  6. Slot Online Terbaru
  7. Situs Game Slot Online Terbaik
  8. Daftar Situs Judi Slot Online Gacor Gampang Menang 2022
  9. Situs Slot Deposit Pulsa dan Slot Online Terbaik
  10. Slot Hacker
  11. Situs Slot Online Terbaik
  12. Slot Gacor Gampang Menang
  13. Situs Judi Slot Online Gacor Terbaik 2022
  14. Situs Judi Slot Online Jackpot Terbesar
  15. Slot Online Terpercaya
  16. Game Slot Penghasil Uang
  17. Situs Slot Online Terbaik 2021
  18. Situs Slot Online Terbaik dan Terpercaya
  19. Situs Slot Terbaik
  20. Situs Judi Slot Terbaik Dan Terpercaya No 1

Abstract

ANTIPROLIFERATIVE EFFECT OF EXTRACTS AND FLAVONOIDS OF Juniperus PhoeniceaL. GROWING IN EGYPT

Author(s): MAII A. I. MAAMOUN 1*, SALMA A. EL-SAWI 1, HEMAIA M. MOTAWAE 1, MOHAMED A. SLEEM2, ABDEL-RAHMAN O. EL-SHABRAWY2, HAWASW. USAMA3AND AYMAN A. FARGHALY

Objective: To Investigate the Cytotoxic effect of the plant Juniperus phoeniceaL. growing inEgypt.Main outcome measures: SRB and MTT assays are used to measure the antiproliferative activity of crude and successive extracts as well as the isolated flavonoids. Also, in vivocytogenetic assays were carried out. Results: The antiproliferative effect of leaves crude extract of Juniperus phoeniceaL. done by SRB assay showed high activity against lung carcinoma (H460), liver tumor (HEPG2) and breast carcinoma (MCF7) cell lines (IC50= 0.6, 0.9 and 4.9μg/mL,  respectively). Consecutively, among the successive extracts of leaves, the ethyl acetate fraction was the most active against (H460) (IC50=7.29μg/mL). On the other hand, the antiproliferative effect of berries crude extract of J. phoeniceadone by MTT assay showed 21% lethality at conc. 100 ppm against HEPG2. Phytochemical investigation of J. phoenicearesulted in the isolation and identification of 4 flavonoids; isoetin-7-O-β-glucoside which was isolated for the first time  from  berries  extract,  isoscutellarein,  amentoflavone  and agathisflavonewhich  was  isolated  for  the  first  time  from  leaves  and  berries  extracts.  Investigating  the antiproliferative  activity  of  the  isolated  flavonoids  by  MTT  assay  revealed  that agathisflavonerecorded  high  activity  against  HEPG2,  colon  carcinoma  (HC112)  and  (H460)(IC50 =3.5, 53.1 and 59.9 μg/mL, respectively). In vivocytogenetic assays (Micronucleus and Chromosome aberrations tests) were carried out on agathisflavoneshowed that no significant differences between treated and negative control groups were observed in the Micronucleus level in polychromatic erythrocytes and in chromosome aberrations in  both  tests,  respectively,  which  indicates  the  safety  of agathisflavoneon  normal  cells.Conclusion: Juniperus phoenicealeaves  exhibited  more  efficient  antiproliferative activity than berries. Also, the biflavone; agathisflavone, isolated for the first time from leaves and berries showedthepotentantiproliferative effect on cancer cell lines and is safe for normal cells of mice under the experimental conditions

Get the App