Mintage Journal of Pharmaceutical and Medical Sciences (ISSN: 2320-3315)

Author(s): PRASANTA KUMAR MITRA

Objective:To know the antibacterial activity of  a compound (AV-2) isolated from the  leaves of  Titeypati (Artemisia vulgaris Linn.),  a medicinal  plants of  North  Eastern Himalaya, against  four Gram-negative bacteria like Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa and Salmonella typhi. as well as four Gram -positive  bacteria  viz.  Bacillius    subtilis,    Bacilus   megaterium,    Staphylococcus aureus   and    Streptococcus   pyogenes. Method:Disc  diffusion    technique   was   used   for   in   vitro  antibacterial  screening.  Results:Result  showed  that AV-2   had  large  zone  of  inhibition  in  disc  diffusion against the said  bacteria. Antibacterial activity was more in Gram -positive  bacteria  than Gram -negative bacteria. Highest  activity  was  noted  against  Bacillus  subtilis  and  lowest  was  found  for  Salmonella  typhi.  The MIC  (minimum inhibitory  concentration)  values  ofAV-2  against  the  bacteriaranged  from 4–32  microgram/mL. Conclusion:AV-2,  thus,  hadwell  anti bacterial activityagainst  the tested bacteria